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验证数据展示

  • WB analysis using 66059-1-Ig (same clone as 66059-1-PBS)

    WB analysis using 66059-1-Ig (same clone as 66059-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66059-1-Ig (same clone as 66059-1-PBS)

    IHC staining of human breast cancer using 66059-1-Ig (same clone as 66059-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15 antibody included
  • IF Staining of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

    IF Staining of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (66059-1-Ig, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66059-1-Ig (same clone as 66059-1-PBS)

    IP experiment of HEK-293 using 66059-1-Ig (same clone as 66059-1-PBS)

    IP result of anti-RBM15 (IP:66059-1-Ig, 3ug; Detection:66059-1-Ig 1:500) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

    WB analysis of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK293 cells using 66059-1-Ig (same clone as 66059-1-PBS)

    WB analysis of HEK293 cells using 66059-1-Ig (same clone as 66059-1-PBS)

    WB result of RBM15 antibody (66059-1-Ig, 1:4000) with si-control and si-RBM15 transfected HEK293 cells. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51442-1

    Cytometric bead array sample test of MP51442-1

    Cytometric bead array sample test of MP51442-1, RBM15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66059-1-PBS. Detection antibody: 66059-2-PBS.

  • Cytometric bead array standard curve of MP51442-1

    Cytometric bead array standard curve of MP51442-1

    Cytometric bead array standard curve of MP51442-1, RBM15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66059-1-PBS. Detection antibody: 66059-2-PBS. Standard:Ag17970. Range: 0.391-100 ng/mL.

  • Featured Product
  • KD/KO验证

RBM15 Monoclonal antibody, PBS Only (Capture)

RBM15 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 66059-1-PBS

产品说明书

CloneNo. 4A1A4

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

别名

4A1A4, One-twenty two protein 1, OTT, OTT1, RBM 15

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RBM15 Ready-To-Use IHC Kit. RBM15 primary antibody included.

产品信息

66059-1-PBS targets RBM15 as part of a matched antibody pair:

MP51442-1: 66059-1-PBS capture and 66059-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17970

Product name: Recombinant human RBM15 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 658-957 aa of BC006397

Sequence: SSSRDRYNSDNDRSSRLLLERPSPIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQDGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQPKLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGGNKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRGFGFQIGVRYENKKRENLALTLL

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 15
别名 4A1A4, One-twenty two protein 1, OTT, OTT1, RBM 15
计算分子量 105 kDa
观测分子量 107 kDa
GenBank蛋白编号BC006397
基因名称 RBM15
Gene ID (NCBI) 64783
RRIDAB_11042326
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96T37
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.