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验证数据展示

  • FC experiment of human PBMCs using 65562-1-MR

    FC experiment of human PBMCs using 65562-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 (4G7) Mouse IgG2a RecAb (65562-1-MR, Clone: 4G7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65562-1-MR

    FC experiment of human PBMCs using 65562-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 (4G7) Mouse IgG2a RecAb (65562-1-MR, Clone: 4G7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody

CD19 Uni-rAb® Recombinant Antibody for FC
Cat No. 65562-1-MR

产品说明书

CloneNo. 4G7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

4G7, B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65562-1-MR targets CD19 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

Human chronic lymphocytic leukemia (CLL) cells

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD19 molecule
别名 4G7, B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4
计算分子量 556 aa, 61 kDa
GenBank蛋白编号BC006338
基因名称 CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
RRIDAB_3670316
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP15391
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

实验方案

Product Specific Protocols
FC protocol for CD19 antibody 65562-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols