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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65147-1-Ig

    FC experiment of human PBMCs using 65147-1-Ig

    1X10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (right), or Mouse IgG1 Isotype Control (left). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65147-1-Ig

    FC experiment of human PBMCs using 65147-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

Anti-Human CD4 (SK3)

CD4 Monoclonal Antibody for FC
Cat No. 65147-1-Ig

产品说明书

CloneNo. SK3

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

CD4, CD4 molecule, CD4mut, SK3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65147-1-Ig targets CD4 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=8.82 x 10-11M
KOff=2.00 x 10-5M
KOn=2.26 x 105M
全称 CD4 molecule
别名 CD4, CD4 molecule, CD4mut, SK3
计算分子量 55 kDa
GenBank蛋白编号BC025782
基因名称 CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_2918432
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for CD4 antibody 65147-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols