验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, NIH/3T3 cells, HeLa cells, K-562 cells, MJ cells, RKO cells, pig liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60952-3-Ig targets GFM2 in WB, ELISA applications and shows reactivity with human, mouse, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag36976 Product name: Recombinant human GFM2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 430-779 aa of BC015712 Sequence: GNIALTVGLKHTATGDTIVSSKSSALAAARRAEREGEKKHRQNNEAERLLLAGVEIPEPVFFCTIEPPSLSKQPDLEHALKCLQREDPSLKVRLDPDSGQTVLCGMGELHIEIIHDRIKREYGLETYLGPLQVAYRETILNSVRATDTLDRTLGDKRHLVTVEVEARPIETSSVMPVIEFEYAESINEGLLKVSQEAIENGIHSACLQGPLLGSPIQDVAITLHSLTIHPGTSTTMISACVSRCVQKALKKADKQVLEPLMNLEVTVARDYLSPVLADLAQRRGNIQEIQTRQDNKVVIGFVPLAEIMGYSTVLRTLTSGSATFALELSTYQAMNPQDQNTLLNRRSGLT 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | G elongation factor, mitochondrial 2 |
| 别名 | EC:3.6.5.-, EF G2mt, EFG2, EFG2mt, EF-G2mt |
| 计算分子量 | 779 aa, 87 kDa |
| 观测分子量 | 87 kDa |
| GenBank蛋白编号 | BC015712 |
| 基因名称 | GFM2 |
| Gene ID (NCBI) | 84340 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q969S9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GFM2 antibody 60952-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
