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验证数据展示

  • WB analysis using 60494-1-Ig

    WB analysis using 60494-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60494-1-Ig (DCP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60494-1-Ig

    WB analysis using 60494-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60494-1-Ig (DCP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 60494-1-Ig

    WB analysis of HEK-293 using 60494-1-Ig

    WB result of DCP1A antibody (60494-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCP1A transfected HEK-293 cells.

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  • KD/KO验证

DCP1A Monoclonal antibody

DCP1A Monoclonal Antibody for WB, ELISA
Cat No. 60494-1-Ig

产品说明书

CloneNo. 2D11E2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, ELISA

别名

2D11E2, EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLoVo cells, HEK-293 cells, rat brain tissue, HCT 116 cells, Jurkat cells, MOLT-4 cells, Raji cells, mouse brain tissue, pig brain tissue
Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60494-1-Ig targets DCP1A in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag18706

Product name: Recombinant human DCP1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC007439

Sequence: MEALSRAGQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQLSNLGSTETLEEMPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHHSVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVLSPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DCP1 decapping enzyme homolog A (S. cerevisiae)
别名 2D11E2, EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A
计算分子量 582 aa, 63 kDa
观测分子量70 kDa
GenBank蛋白编号BC007439
基因名称 DCP1A
Gene ID (NCBI) 55802
RRIDAB_3670263
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

实验方案

Product Specific Protocols
WB protocol for DCP1A antibody 60494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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