验证数据展示
经过测试的应用
| Positive WB detected in | human colon tissue, pig colon tissue |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue, human colon tissue, human stomach cancer tissue, human colorectal cancer tissue, human large intestine tissue, human small intestine tissue, human appendicitis tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
60351-1-Ig targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag19488 Product name: Recombinant human CDH17 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 89-406 aa of BC113464 Sequence: RSTHNLQVAALDANGIIVEGPVPITIEVKDINDNRPTFLQSKYEGSVRQNSRPGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLVISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLPRFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cadherin 17, LI cadherin (liver-intestine) |
| 别名 | CDH17, 3B10A9, Cadherin 17, CDH16, HPT 1 |
| 计算分子量 | 832 aa, 92 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC113464 |
| 基因名称 | Cadherin-17 |
| Gene ID (NCBI) | 1015 |
| RRID | AB_2881460 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q12864 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Cadherin-17 antibody 60351-1-Ig | Download protocol |
| IHC protocol for Cadherin-17 antibody 60351-1-Ig | Download protocol |
| WB protocol for Cadherin-17 antibody 60351-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
