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验证数据展示

  • WB analysis of fetal human brain using 60336-1-Ig

    WB analysis of fetal human brain using 60336-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 60336-1-Ig

    IF Staining of MCF-7 using 60336-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 60336-1-Ig(DBC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

BRINP1 Monoclonal antibody

BRINP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 60336-1-Ig

产品说明书

CloneNo. 2A10E3

宿主/亚型

Mouse / IgG2c

种属反应性

human, pig, mouse

应用

WB, IF/ICC, ELISA

别名

BRINP1, DBC1, DBCCR1, deleted in bladder cancer 1, FAM5A, Protein FAM5A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected infetal human brain tissue, mouse brain tissue, pig brain tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60336-1-Ig targets BRINP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, pig, mouse
免疫原

CatNo: Ag6588

Product name: Recombinant human DBC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-306 aa of BC021560

Sequence: MNWRFVELLYFLFIWGRISVQPSHQEPAGTDQHVSKEFDWLISDRGPFHHSRSYLSFVERHRQGFTTRYKIYREFARWKVRNTAIERRDLVRHPVPLMPEFQRSIRLLGRRPTTQQFIDTIIKKYGTHLLISATLGGEEALTMYMDKSRLDRKSGNATQSVEALHQLASSYFVDRDGTMRRLHEIQISTGAIKVTETRTGPLGCNSYDNLDSVSSVLLQSTESKLHLQGLQIIFPQYLQEKFVQSALSYIMCNGEGEYLCQNSQCRCQCAEEFPQCNCPITDIQIMEYTLANMAKSWAEAYKDLEN

种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 deleted in bladder cancer 1
别名 BRINP1, DBC1, DBCCR1, deleted in bladder cancer 1, FAM5A, Protein FAM5A
计算分子量 761 aa, 88 kDa
观测分子量 88 kDa
GenBank蛋白编号BC021560
基因名称 BRINP1
Gene ID (NCBI) 1620
RRIDAB_2881445
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60477
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRINP1, also known as DBC1 and FAM5A, inhibits cell proliferation by negative regulation of the G1/S transition. It mediates cell death which is not of the classical apoptotic type and regulates expression of components of the plasminogen pathway.

实验方案

Product Specific Protocols
IF protocol for BRINP1 antibody 60336-1-IgDownload protocol
WB protocol for BRINP1 antibody 60336-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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