验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60264-1-Ig targets CCT3 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0843 Product name: Recombinant human CCT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 246-545 aa of BC006501 Sequence: EYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chaperonin containing TCP1, subunit 3 (gamma) |
| 别名 | TCP 1 gamma, 5F5A6, CCT gamma, CCTG, CCTgamma |
| 计算分子量 | 60 kDa |
| 观测分子量 | 56-60 kDa |
| GenBank蛋白编号 | BC006501 |
| 基因名称 | CCT3 |
| Gene ID (NCBI) | 7203 |
| RRID | AB_2881385 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49368 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCT3 protein (full name: chaperonin containing TCP1 subunit 3, also known as the gamma subunit of TCP-1 protein) is an important member of the TCP-1 chaperonin family. As one of the eight essential subunits of the CCT/TRiC complex, its core function is to act as a molecular chaperone that assists in the correct folding of other cellular proteins under ATP hydrolysis. It is particularly involved in the folding process of key cytoskeletal components such as actin and tubulin, while also participating in important biological activities including ciliogenesis and telomere maintenance.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CCT3 antibody 60264-1-Ig | Download protocol |
| IHC protocol for CCT3 antibody 60264-1-Ig | Download protocol |
| WB protocol for CCT3 antibody 60264-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins | ||
Front Cell Dev Biol The BBS/CCT chaperonin complex ensures the localization of the adhesion G protein-coupled receptor ADGRV1 to the base of primary cilia |
