验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, pig adipose tissue, Jurkat cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60193-1-Ig targets PPARD in WB, IHC, ChIP, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, mouse, rat, pig, zebrafish |
| 免疫原 |
CatNo: Ag16770 Product name: Recombinant human PPARD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 102-361 aa of BC002715 Sequence: IRMKLEYEKCERSCKIQKKNRNKCQYCRFQKCLALGMSHNAIRFGRMPEAEKRKLVAGLTANEGSQYNPQVADLKAFSKHIYNAYLKNFNMTKKKARSILTGKASHTAPFVIHDIETLWQAEKGLVWKQLVNGLPPYKEISVHVFYRCQCTTVETVRELTEFAKSIPSFSSLFLNDQVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTREFLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLALFIAAIILCGGE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | peroxisome proliferator-activated receptor delta |
| 别名 | PPAR delta, 1B10E1, Peroxisome proliferator-activated receptor beta, Peroxisome proliferator-activated receptor delta, PPAR beta |
| 计算分子量 | 50 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC002715 |
| 基因名称 | PPARD |
| Gene ID (NCBI) | 5467 |
| RRID | AB_10896827 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q03181 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPARD antibody 60193-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Axin formation inhibitor 1 aggravates hepatic ischemia‒reperfusion injury by promoting the ubiquitination and degradation of PPARβ | ||
Genes Dis 8-Chloroadenosine suppresses hepatocellular carcinoma progression via ADAR1/PPARγ axis-mediated lipid metabolism. | ||
Adv Sci (Weinh) The Loss of an Orphan Nuclear Receptor NR2E3 Augments Wnt/β-catenin Signaling via Epigenetic Dysregulation that Enhances Sp1-β catenin-p300 Interactions in Hepatocellular Carcinoma | ||
Cell Death Dis CILP1 interacting with YBX1 promotes hypertrophic scar formation by suppressing PPARs transcription | ||
Clin Transl Med Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma |
