验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, COLO 320 cells, bEnd.3 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60170-1-Ig targets TEM1/CD248 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag13334 Product name: Recombinant human CD248 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 399-687 aa of BC051340 Sequence: EPTQPPDFALAYRPSFPEDREPQIPYPEPTWPPPLSAPRVPYHSSVLSVTRPVVVSATHPTLPSAHQPPVIPATHPALSRDHQIPVIAANYPDLPSAYQPGILSVSHSAQPPAHQPPMISTKYPELFPAHQSPMFPDTRVAGTQTTTHLPGIPPNHAPLVTTLGAQLPPQAPDALVLRTQATQLPIIPTAQPSLTTTSRSPVSPAHQISVPAATQPAALPTLLPSQSPTNQTSPISPTHPHSKAPQIPREDGPSPKLALWLPSPAPTAAPTALGEAGLAEHSQRDDRWL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD248 molecule, endosialin |
| 别名 | CD248, TEM1, 1F9B4, CD164L1, CD248 molecule, endosialin |
| 计算分子量 | 757 aa, 81 kDa |
| 观测分子量 | 150-160 kDa |
| GenBank蛋白编号 | BC051340 |
| 基因名称 | TEM1 |
| Gene ID (NCBI) | 57124 |
| RRID | AB_10694834 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9HCU0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TEM1/CD248 antibody 60170-1-Ig | Download protocol |
| WB protocol for TEM1/CD248 antibody 60170-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer | ||
Nat Commun Targeted therapies of inflammatory diseases with intracellularly gelated macrophages in mice and rats | ||
Adv Sci (Weinh) Understanding Glioblastoma Dynamics Using 3D Organoids and Engineered Extracellular Matrix. | ||
Nat Cardiovasc Res Dynamic molecular atlas of cardiac fibrosis at single-cell resolution shows CD248 in cardiac fibroblasts orchestrates interactions with immune cells
| ||
EBioMedicine CD248 promotes insulin resistance by binding to the insulin receptor and dampening its insulin-induced autophosphorylation
| ||
