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验证数据展示

  • IHC staining of mouse brain using 33901-1-AP

    IHC staining of mouse brain using 33901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33901-1-AP (PLP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 33901-1-AP

    IHC staining of human brain using 33901-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 33901-1-AP (PLP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 33901-1-AP

    IHC staining of rat brain using 33901-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 33901-1-AP (PLP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 33901-1-AP

    IHC staining of rat brain using 33901-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 33901-1-AP (PLP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 33901-1-AP

    IF Staining of mouse brain using 33901-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PLP1 antibody (33901-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 33901-1-AP

    IF Staining of mouse brain using 33901-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PLP1 antibody (33901-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CoraLite® Plus 488 MBP antibody (CL488-10458, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLP1 Polyclonal antibody

PLP1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 33901-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, ELISA

别名

Lipophilin, Myelin proteolipid protein, PLP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33901-1-AP targets PLP1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag38738

Product name: Recombinant human PLP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 89-151 aa of NM_000533.4

Sequence: EGFYTTGAVRQIFGDYKTTICGKGLSATVTGGQKGRGSRGQHQAHSLERVCHCLGKWLGHPDK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteolipid protein 1
别名 Lipophilin, Myelin proteolipid protein, PLP
计算分子量30kDa，277aa
GenBank蛋白编号NM_000533.4
基因名称 PLP1
Gene ID (NCBI) 5354
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP60201
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myelin proteolipid protein is also named PLP1 and Lipophilin. Plp1 is abundantly expressed by oligodendrocytes of the central nervous system (CNS), where it encodes the most abundant protein in CNS myelin (PMID: 19452287). Plp1 expression preferentially occurs during early postnatal development, primarily as the DM20 isoform (PMID: 37293625).

实验方案

Product Specific Protocols
IF protocol for PLP1 antibody 33901-1-APDownload protocol
IHC protocol for PLP1 antibody 33901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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