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验证数据展示

  • WB analysis using 29846-1-AP

    WB analysis using 29846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29846-1-AP (STK24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29846-1-AP

    WB analysis using 29846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29846-1-AP (STK24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29846-1-AP

    IHC staining of mouse testis using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK24 antibody included
  • IHC staining of mouse testis using 29846-1-AP

    IHC staining of mouse testis using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK24 antibody included
  • IHC staining of mouse colon using 29846-1-AP

    IHC staining of mouse colon using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK24 antibody included
  • IHC staining of human placenta using 29846-1-AP

    IHC staining of human placenta using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK24 antibody included

STK24 Polyclonal antibody

STK24 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29846-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EC:2.7.11.1, Mammalian STE20-like protein kinase 3, MST3, Serine/threonine-protein kinase 24 12 kDa subunit, Serine/threonine-protein kinase 24 36 kDa subunit

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse lung tissue, HeLa cells, Jurkat cells, rat lung tissue, rat skin tissue, rat spleen tissue
Positive IHC detected inmouse testis tissue, mouse colon tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® STK24 Ready-To-Use IHC Kit. STK24 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29846-1-AP targets STK24 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31519

Product name: Recombinant human STK24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 320-443 aa of NM_003576

Sequence: SDAETDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine/threonine kinase 24 (STE20 homolog, yeast)
别名 EC:2.7.11.1, Mammalian STE20-like protein kinase 3, MST3, Serine/threonine-protein kinase 24 12 kDa subunit, Serine/threonine-protein kinase 24 36 kDa subunit
计算分子量49KD
观测分子量50 kDa
GenBank蛋白编号NM_003576
基因名称 STK24
Gene ID (NCBI) 8428
RRIDAB_2923614
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6E0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).

实验方案

Product Specific Protocols
IHC protocol for STK24 antibody 29846-1-APDownload protocol
WB protocol for STK24 antibody 29846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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