验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27327-1-AP targets DUSP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25380 Product name: Recombinant human DUSP2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-314 aa of BC007771 Sequence: MGLEAARELECAALGTLLRDPREAERTLLLDCRPFLAFCRRHVRAARPVPWNALLRRRARGPPAAVLACLLPDRALRTRLVRGELARAVVLDEGSASVAELRPDSPAHVLLAALLHETRAGPTAVYFLRGGFDGFQGCCPDLCSEAPAPALPPTGDKTSRSDSRAPVYDQGGPVEILPYLFLGSCSHSSDLQGLQACGITAVLNVSASCPNHFEGLFRYKSIPVEDNQMVEISAWFQEAIGFIDWVKNSGGRVLVHCQAGISRSATICLAYLMQSRRVRLDEAFDFVKQRRGVISPNFSFMGQLLQFETQVLCH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dual specificity phosphatase 2 |
| 别名 | dual specificity phosphatase 2, DUSP2, PAC 1, PAC1 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC007771 |
| 基因名称 | DUSP2 |
| Gene ID (NCBI) | 1844 |
| RRID | AB_2880845 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q05923 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DUSP2 belongs to the sub-family of nuclear specific DUSPs that includes DUSP1, 4 and 5. DUSP2 is highly expressed in immune cells, in particular, of patients with inflammatory arthritis, such as in rheumatoid arthritis (RA), and Dusp2 knockout mice present a significant reduction in inflammatory responses. It has been demonstrated that DUSP2 is a downstream target of the tumor suppressor p53 that is required for the cellular response to oxidative stress. DUSP2 is also involved in transcription factor E2F1-mediated apoptotic signaling. Like other members of the DUSP family, DUSP2 interacts with MAPKs; However, the target protein for its phosphatase activity is controversial.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DUSP2 antibody 27327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity | ||
Nat Commun Phosphoglycerate dehydrogenase-mediated serine reprogramming aggravates macrophage hyperinflammation in murine Pseudomonas aeruginosa pneumonia. | ||
Cell Death Differ Retinoblastoma-associated protein is important for TRIM24-mediated activation of the mTOR signaling pathway through DUSP2 action in prostate cancer | ||
Cell Death Dis NY-ESO-1 facilitates anoikis resistance and tumor metastasis by hijacking deubiquitinase OTUB1 to stabilize PP1α. | ||
Naunyn Schmiedebergs Arch Pharmacol FBXO45 enhances cell viability and glycolysis in cervical cancer via DUSP2 ubiquitination-mediated ERK1/2 activation |
