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验证数据展示

  • WB analysis of Recombinant protein using 22646-1-AP

    WB analysis of Recombinant protein using 22646-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 22646-1-AP (IL-28A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 22646-1-AP

    IHC staining of human tonsillitis using 22646-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22646-1-AP (IL-28A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 22646-1-AP

    IHC staining of human tonsillitis using 22646-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22646-1-AP (IL-28A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-28A Polyclonal antibody

IL-28A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22646-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRecombinant protein protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22646-1-AP targets IL-28A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18525

Product name: Recombinant human IL-28A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-91 aa of BC113583

Sequence: TVTGAVPVARLHGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 28A (interferon, lambda 2)
别名 Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20
计算分子量 200 aa, 22 kDa
GenBank蛋白编号BC113583
基因名称 IL-28A
Gene ID (NCBI) 282616
RRIDAB_2879141
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.

实验方案

Product Specific Protocols
WB protocol for IL-28A antibody 22646-1-APDownload protocol
IHC protocol for IL-28A antibody 22646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols