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验证数据展示

  • WB analysis using 21138-1-AP

    WB analysis using 21138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IF Staining of HepG2 using 21138-1-AP

    IF Staining of HepG2 using 21138-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 21138-1-AP

    IP experiment of HeLa using 21138-1-AP

    IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug.

  • IHC staining of mouse lung using 21138-1-AP

    IHC staining of mouse lung using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IHC staining of mouse ovary using 21138-1-AP

    IHC staining of mouse ovary using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IHC staining of mouse ovary using 21138-1-AP

    IHC staining of mouse ovary using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IHC staining of human ovary cancer using 21138-1-AP

    IHC staining of human ovary cancer using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IHC staining of mouse ovary using 21138-1-AP

    IHC staining of mouse ovary using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IHC staining of mouse ovary using 21138-1-AP

    IHC staining of mouse ovary using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included

KIAA0528 Polyclonal antibody

KIAA0528 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21138-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

C2 domain-containing phosphoprotein of 138 kDa, C2 domain-containing protein 5, C2CD5, CDP138

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse small intestine tissue, mouse lung tissue, human ovary cancer tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy C2CD5 Ready-To-Use IHC Kit. C2CD5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21138-1-AP targets KIAA0528 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15590

Product name: Recombinant human KIAA0528 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 651-1000 aa of BC042498

Sequence: RSRLLRSQSESSDEVTELDLSHGKKDAFVLEIDDTDAMEDVHSLLTDVPPPSGFYSCNTEIMPGINNWTSEIQMFTSVRVIRLSSLNLTNQALNKNFNDLCENLLKSLYFKLRSMIPCCLCHVNFTVSLPEDELIQVTVTAVAITFDKNQALQTTKTPVEKSLQRASTDNEELLQFPLELCSDSLPSHPFPPAKAMTVEKASPVGDGNFRNRSAPPCANSTVGVVKMTPLSFIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIAEVFAMVRAHVAALGGNAVVSYIMKQCVFMENPNKNQAQCLINVSGDAVVFVRESDLEVVSSQQPTTNCQSSCTEGEVTT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0528
别名 C2 domain-containing phosphoprotein of 138 kDa, C2 domain-containing protein 5, C2CD5, CDP138
计算分子量 1000 aa, 110 kDa
观测分子量 110-125 kDa
GenBank蛋白编号BC042498
基因名称 KIAA0528
Gene ID (NCBI) 9847
RRIDAB_10858480
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86YS7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for KIAA0528 antibody 21138-1-APDownload protocol
IHC protocol for KIAA0528 antibody 21138-1-APDownload protocol
IP protocol for KIAA0528 antibody 21138-1-APDownload protocol
WB protocol for KIAA0528 antibody 21138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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