验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, NCI-H1299 cells, HT-29 cells, RKO cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy® PAPSS2 Ready-To-Use IHC Kit. PAPSS2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
15978-1-AP targets PAPSS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8766 Product name: Recombinant human PAPSS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC009894 Sequence: MSGIKKQKTENQQKSTNVVYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTISFALEEYLVSHAIPCYSLDGDNVRHGLNRNLGFSPGDREENIRRIAEVAKLFADAGLVCITSFISPFAKDRENARKIHESAGLPFFEIFVDAPLNICESRDVKGLYKRARAGEIKGFTGIDSDYEKPETPERVLKTNLSTVSDCVHQVVELLQEQNIVPYTIIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDTLLDDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 3'-phosphoadenosine 5'-phosphosulfate synthase 2 |
| 别名 | 3'-phosphoadenosine-5'-phosphosulfate synthase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, Adenylyl-sulfate kinase, ATPSK2 |
| 计算分子量 | 614 aa, 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC009894 |
| 基因名称 | PAPSS2 |
| Gene ID (NCBI) | 9060 |
| RRID | AB_2159498 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95340 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAPSS2 antibody 15978-1-AP | Download protocol |
| WB protocol for PAPSS2 antibody 15978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Neuroprotective response against the onset of ischemic stroke by upregulation of histone H3Y99 sulfation. | ||
Hum Reprod PAPSS2-mediated BMPR1A sulfation in stromal cells regulating endometrial decidualization via autophagy. | ||
Discov Oncol Application of Mendelian randomization and bioinformatic analysis to construct a prognostic model for thyroid cancer and perform pan-cancer analysis |
