验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse brain tissue, mouse liver tissue, rat liver tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14575-1-AP targets SDHC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rabbit |
| 免疫原 |
CatNo: Ag5775 Product name: Recombinant human SDHC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-169 aa of BC033626 Sequence: MAALLLRHVGRHCLRAHFSPQLCIRNAVPLGTTAKEEMERFWNKNIGSNRPLSPHITIYSWSLPMAMSICHRGTGIALSAGVSLFGMSALLLPGNFESYLELVKSLCLGPALIHTAKFALVFPLMYHTWNGIRHLMWDLGKGLKIPQLYQSGVVVLVLTVLSSMGLAAM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa |
| 别名 | CYB560, CYBL, PGL3, QPs 1, QPS1, SDH3, SDHC |
| 计算分子量 | 19 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC033626 |
| 基因名称 | SDHC |
| Gene ID (NCBI) | 6391 |
| RRID | AB_2183291 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99643 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDHC(Succinate dehydrogenase cytochrome b560 subunit, mitochondrial) is also named as CYB560, SDH3 and belongs to the cytochrome b560 family. It is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3) and paraganglioma and gastric stromal sarcoma (PGGSS). It has a transit peptide of 29 amino acid and has 5 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SDHC antibody 14575-1-AP | Download protocol |
| WB protocol for SDHC antibody 14575-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Iron-addicted colorectal cancers exploit heme-complex II axis to resist oxidative cell death.
| ||
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. | ||
Nucleic Acids Res Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice. | ||
Sci Adv Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer | ||
Pharmacol Res Discovery of KMT5A repressed miR-99b cluster with potential to restore chemotherapy sensitivity in gastric cancer by regulating mitochondrial complex II and affecting OXPHOS. |
