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验证数据展示

  • WB analysis using 13690-1-AP

    WB analysis using 13690-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of C6 using 13690-1-AP

    IP experiment of C6 using 13690-1-AP

    IP result of anti-NEDD4L (IP:13690-1-AP, 4ug; Detection:13690-1-AP 1:300) with C6 cells lysate 1000 ug.

  • IHC staining of human stomach cancer using 13690-1-AP

    IHC staining of human stomach cancer using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IF Staining of PC-3 using 13690-1-AP

    IF Staining of PC-3 using 13690-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (13690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse kidney using 13690-1-AP

    IHC staining of mouse kidney using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IHC staining of human placenta using 13690-1-AP

    IHC staining of human placenta using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IHC staining of human stomach cancer using 13690-1-AP

    IHC staining of human stomach cancer using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IHC staining of mouse stomach using 13690-1-AP

    IHC staining of mouse stomach using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IHC staining of rat kidney using 13690-1-AP

    IHC staining of rat kidney using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IHC staining of rat stomach using 13690-1-AP

    IHC staining of rat stomach using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IHC staining of human stomach cancer using 13690-1-AP

    IHC staining of human stomach cancer using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IHC staining of rat kidney using 13690-1-AP

    IHC staining of rat kidney using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • IHC staining of rat stomach using 13690-1-AP

    IHC staining of rat stomach using 13690-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD4L antibody included
  • Featured Product
  • KD/KO Validated

NEDD4L Polyclonal antibody

NEDD4L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13690-1-AP

产品说明书

引用文献(73)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

E3 ubiquitin-protein ligase NEDD4-like, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NED4L, hNEDD4-2, KIAA0439

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inC6 cells, HT-1080 cells, PC-3 cells, PC-12 cells
Positive IP detected inC6 cells
Positive IHC detected inhuman stomach cancer tissue, mouse kidney tissue, rat stomach tissue, human placenta tissue, human stomach cancer tissue, rat kidney tissue, mouse stomach tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy NEDD4L Ready-To-Use IHC Kit. NEDD4L primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13690-1-AP targets NEDD4L in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原

CatNo: Ag4565

Product name: Recombinant human NEDD4L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 565-911 aa of BC032597

Sequence: EESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 4-like
别名 E3 ubiquitin-protein ligase NEDD4-like, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NED4L, hNEDD4-2, KIAA0439
计算分子量 854 aa, 96 kDa
观测分子量 112 kDa, 125 kDa
GenBank蛋白编号BC032597
基因名称 NEDD4L
Gene ID (NCBI) 23327
RRIDAB_2149326
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

实验方案

Product Specific Protocols
IF protocol for NEDD4L antibody 13690-1-APDownload protocol
IHC protocol for NEDD4L antibody 13690-1-APDownload protocol
IP protocol for NEDD4L antibody 13690-1-APDownload protocol
WB protocol for NEDD4L antibody 13690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Hepatol

eIF3f promotes tumour malignancy by remodelling fatty acid biosynthesis in hepatocellular carcinoma

Authors - Suiqing Zhou
View Article
humanWB,IHC

Cancer Commun (Lond)

Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer

Authors - Yan Guo
View Article
humanWB,IHC,IF

Nat Genet

Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.

Authors - Loïc Broix
View Article
mouse,humanWB

Nat Commun

Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes.

Authors - Chuqi Liu
View Article
humanWB

Nat Commun

Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets.

Authors - Aline Lieber
View Article
mouse,humanWB,IF

Nat Commun

Endothelial major vault protein alleviates vascular remodeling via promoting Parkin-mediated mitophagy

Authors - Bin Jiang
View Article
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