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验证数据展示

  • IF Staining of HepG2 using 12420-1-AP

    IF Staining of HepG2 using 12420-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12420-1-AP (Septin 10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human kidney using 12420-1-AP

    WB analysis of human kidney using 12420-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12420-1-AP (Septin 10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12420-1-AP

    IHC staining of human pancreas cancer using 12420-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12420-1-AP (Septin 10 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SEPT10 antibody included
  • IHC staining of mouse kidney using 12420-1-AP

    IHC staining of mouse kidney using 12420-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12420-1-AP (Septin 10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPT10 antibody included
  • IHC staining of human placenta using 12420-1-AP

    IHC staining of human placenta using 12420-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12420-1-AP (Septin 10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPT10 antibody included
  • IHC staining of rat heart using 12420-1-AP

    IHC staining of rat heart using 12420-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 12420-1-AP (Septin 10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPT10 antibody included
  • IHC staining of rat kidney using 12420-1-AP

    IHC staining of rat kidney using 12420-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12420-1-AP (Septin 10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPT10 antibody included

Septin 10 Polyclonal antibody

Septin 10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12420-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SEPT10, SEPTIN10, Septin-10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman kidney tissue, HeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human placenta tissue, rat kidney tissue, rat heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® SEPT10 Ready-To-Use IHC Kit. SEPT10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12420-1-AP targets Septin 10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag3071

Product name: Recombinant human SEPT10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC020502

Sequence: MASSEVARHLLFQSHMATKTTCMSSQGSDDEQIKRENIRSLTMSGHVGFESLPDQLVNRSIQQGFCFNILCVGETGIGKSTLIDTLFNTNFEDYESSHFCPNVKLKAQTYELQESNVQLKLTIVNTVGFGDQINKEESYQPIVDYIDAQFEAYLQEELKIKRSLFTYHDSRIHVCLYFISPTGHSLKTLDLLTMKNLDSKVNIIPVIAKADTVSKTELQKFKIKLMSELVSNGVQIYQFPTDDDTIAKVNAAMNGQLPFAVVGSMDEVKVGNKMVKARQYPWGVVQVENENHCDFVKLREMLICTNMEDLREQTHTRHYELYRRCKLEEMGFTDVGPENKPVSVQETYEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 septin 10
别名 SEPT10, SEPTIN10, Septin-10
计算分子量 53 kDa, 57 kDa
观测分子量 53-57 kDa
GenBank蛋白编号BC020502
基因名称 Septin 10
Gene ID (NCBI) 151011
RRIDAB_2186992
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P0V9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Septin 10 antibody 12420-1-APDownload protocol
IHC protocol for Septin 10 antibody 12420-1-APDownload protocol
WB protocol for Septin 10 antibody 12420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

RNA

Positioning of sperm tail longitudinal columns depends on NSUN7, an RNA-binding protein destabilizing elongated spermatid transcripts.

Authors - Ekaterina A. Guseva
View Article
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