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验证数据展示

  • IP experiment of HepG2 using 11927-1-AP

    IP experiment of HepG2 using 11927-1-AP

    IP result of anti-ERGIC2 (IP:11927-1-AP, 4ug; Detection:11927-1-AP 1:1000) with HepG2 cells lysate 2900ug.

  • IHC staining of human prostate cancer using 11927-1-AP

    IHC staining of human prostate cancer using 11927-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 11927-1-AP

    IF Staining of HepG2 using 11927-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 11927-1-AP

    WB analysis using 11927-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11927-1-AP (ERGIC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 11927-1-AP

    WB analysis of HeLa using 11927-1-AP

    WB result of ERGIC2 antibody (11927-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC2 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

ERGIC2 Polyclonal antibody

ERGIC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11927-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PTX1, ERV41, ERGIC and golgi 2, Endoplasmic reticulum-Golgi intermediate compartment protein 2, CDA14

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11927-1-AP targets ERGIC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag2603

Product name: Recombinant human ERGIC2 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-377 aa of BC000887

Sequence: MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDFSSKLRINIDITVAMKCQYVGADVLDLAETMVASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSLQDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHESYNFSHRIDHLSFGELVPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISADTHQFSVTERERIINHAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGMLHGIGKFIVEIICCRFRLGSYKPVNSVPFEDGHTDNHLPLLENNTH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ERGIC and golgi 2
别名 PTX1, ERV41, ERGIC and golgi 2, Endoplasmic reticulum-Golgi intermediate compartment protein 2, CDA14
计算分子量 377 aa, 43 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC000887
基因名称 ERGIC2
Gene ID (NCBI) 51290
RRIDAB_2098440
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RQ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERGIC2, also named as Endoplasmic reticulum-Golgi intermediate compartment protein 2 or PTX1, is a 377 amino acid protein, which belongs to the ERGIC family. ERGIC2 is ubiquitously expressed in many tissues. ERGIC2 localizes in the endoplasmic reticulum-Golgi intermediate compartment membrane and may play a role in transport between endoplasmic reticulum and Golgi. ERGIC2 is involved in protein transportation, and participated in the elongation factor 1 alpha regulated mechanisms.

实验方案

Product Specific Protocols
IF protocol for ERGIC2 antibody 11927-1-APDownload protocol
IHC protocol for ERGIC2 antibody 11927-1-APDownload protocol
IP protocol for ERGIC2 antibody 11927-1-APDownload protocol
WB protocol for ERGIC2 antibody 11927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Biol Cell

An integrated proximity labeling and vesicle reconstitution assay identifies novel regulators of Sonic hedgehog secretion.

Authors - Ziyang Song
  • KD Validated
View Article

DNA Cell Biol

Characterization of a Variant of ERGIC2 Transcript.

Authors - Kwok Simon C M SC
View Article
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