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验证数据展示

  • WB analysis of Jurkat using 11031-1-AP

    WB analysis of Jurkat using 11031-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 11031-1-AP

    WB analysis using 11031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11031-1-AP

    IHC staining of mouse testis using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11031-1-AP

    IHC staining of mouse testis using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11031-1-AP

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11031-1-AP

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11031-1-AP

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CPSF1 Polyclonal antibody

CPSF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11031-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CPSF 160 kDa subunit, CPSF1, CPSF160, HSU37012, P/cl.18

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, human brain tissue, HeLa cells
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11031-1-AP targets CPSF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag1489

Product name: Recombinant human CPSF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1039-1443 aa of BC017232

Sequence: STNTPCARIPRMTGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKIFLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGATEGLSKKSVVWENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLDGELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleavage and polyadenylation specific factor 1, 160kDa
别名 CPSF 160 kDa subunit, CPSF1, CPSF160, HSU37012, P/cl.18
计算分子量 160 kDa
观测分子量 160 kDa
GenBank蛋白编号BC017232
基因名称 CPSF1
Gene ID (NCBI) 29894
RRIDAB_2245276
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ10570
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.

实验方案

Product Specific Protocols
IHC protocol for CPSF1 antibody 11031-1-APDownload protocol
WB protocol for CPSF1 antibody 11031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell

Functional atlas of vertebrate viral RNA elements that stabilize RNA and enhance translation.

Authors - Jenny J. Seo
View Article
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
View Article
humanWB

J Biosci

Targeting cleavage and polyadenylation specific factor 1 via shRNA inhibits cell proliferation in human ovarian cancer.

Authors - Beiguang Zhang
  • KD Validated
View Article
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