验证数据展示

IHC staining of mouse brain using 12892-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TDP-43 antibody included
WB analysis using 12892-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 12892-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
WB analysis of A549 cells using 12892-1-AP
A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.
IF Staining of mouse brain using 12892-1-AP
Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IP experiment of HeLa using 12892-1-AP
IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.
FC experiment of HeLa using 12892-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (C-terminal) (12892-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
IHC staining of rat brain using 12892-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TDP-43 antibody included
IF Staining of mouse brain using 12892-1-AP
Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

TDP-43 (C-terminal) Polyclonal antibody

TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

Cat No. 12892-1-AP

产品说明书

引用文献(247)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA and More (4)

别名

TARDBP, TDP 43, TDP43, TDP-43, ALS10

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

Biotin CoraLite® Plus 488 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (247)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, C6 cells, mouse brain tissue, Calu-1 cells, HeLa cells, K-562 cells
Positive IP detected in	HeLa cells, mouse brain tissue
Positive IHC detected in	rat brain tissue, human gliomas tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	HeLa cells, Neuro-2a cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:2000-1:8000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 13 publications below
WB	See 149 publications below
IHC	See 50 publications below
IF	See 92 publications below
IP	See 10 publications below
CoIP	See 2 publications below
ChIP	See 2 publications below
RIP	See 1 publications below

产品信息

12892-1-AP targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, chIP, RIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, monkey, chicken, zebrafish, drosophila
免疫原	fusion protein 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	TAR DNA binding protein
别名	TARDBP, TDP 43, TDP43, TDP-43, ALS10
计算分子量	43 kDa
观测分子量	43-45 kDa, 35 kDa
GenBank蛋白编号	BC001487
基因名称	TDP-43
Gene ID (NCBI)	23435
RRID	AB_2200505
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q13148
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

实验方案

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 12892-1-AP	Download protocol
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-AP	Download protocol
IF protocol for TDP-43 (C-terminal) antibody 12892-1-AP	Download protocol
IP protocol for TDP-43 (C-terminal) antibody 12892-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Nature TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A. Authors - Anna-Leigh Brown View Article
human	WB,IHC	Science HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells. Authors - Haiyang Yu KO Validated View Article
human,mouse	IF	Nature TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle. Authors - Thomas O Vogler View Article
mouse	WB,IF	Nature Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice. Authors - Lindsay A Becker View Article
human	IF	Nat Med Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis. Authors - Payam Mohassel View Article
human	WB	Nat Cell Biol Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition Authors - Shan Lu View Article

验证数据展示

IHC staining of mouse brain using 12892-1-AP

WB analysis using 12892-1-AP

IF Staining of HeLa using 12892-1-AP

WB analysis of A549 cells using 12892-1-AP

IF Staining of mouse brain using 12892-1-AP

IP experiment of HeLa using 12892-1-AP

FC experiment of HeLa using 12892-1-AP

IHC staining of rat brain using 12892-1-AP

IF Staining of mouse brain using 12892-1-AP

TDP-43 (C-terminal) Polyclonal antibody

TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.

TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle.

Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice.

Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis.

Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition