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PYCR2 Polyclonal antibody
PYCR2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17146-1-AP targets PYCR2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, African green monkey |
| Immunogen | PYCR2 fusion protein Ag10918 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | pyrroline-5-carboxylate reductase family, member 2 |
| Synonyms | P5C reductase 2, P5CR 2, P5CR2, PYCR2 |
| Calculated Molecular Weight | 320 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC020553 |
| Gene Symbol | PYCR2 |
| Gene ID (NCBI) | 29920 |
| RRID | AB_2253344 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96C36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PYCR2 antibody 17146-1-AP | Download protocol |
| IHC protocol for PYCR2 antibody 17146-1-AP | Download protocol |
| IF protocol for PYCR2 antibody 17146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis. | ||
Cell Rep Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.
| ||
Virol Sin African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.
|
