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验证数据展示

  • WB analysis using 17146-1-AP

    WB analysis using 17146-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17146-1-AP

    WB analysis using 17146-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17146-1-AP

    IHC staining of human breast cancer using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 17146-1-AP

    IHC staining of human breast cancer using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 17146-1-AP

    IF Staining of HeLa using 17146-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR2 antibody (17146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17146-1-AP

产品说明书

引用文献(33)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

EC:1.5.1.2, HLD10, P5CR 2, P5CR2, PYCR 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, L02 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17146-1-AP targets PYCR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, african green monkey
免疫原

CatNo: Ag10918

Product name: Recombinant human PYCR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC020553

Sequence: MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase family, member 2
别名 EC:1.5.1.2, HLD10, P5CR 2, P5CR2, PYCR 2
计算分子量 320 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC020553
基因名称 PYCR2
Gene ID (NCBI) 29920
RRIDAB_2253344
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96C36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

实验方案

Product Specific Protocols
IF protocol for PYCR2 antibody 17146-1-APDownload protocol
IHC protocol for PYCR2 antibody 17146-1-APDownload protocol
WB protocol for PYCR2 antibody 17146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Nature

Nutrient requirements of organ-specific metastasis in breast cancer.

Authors - Keene L. Abbott
  • KO Validated
View Article

Nature

Metabolite glues as a means of purine sensing and chemotherapeutic response.

Authors - Samuel R. Witus
View Article
humanIF

Cell

Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.

Authors - Qiang Gao
View Article
WB

Nat Metab

Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.

Authors - Diem H Tran
View Article
humanWB

Nat Metab

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Authors - Emily J Kay
View Article
humanWB

Nat Commun

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Authors - Ling Guo
View Article
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