Speedy™ Mouse Tie-2 One-Step ELISA Kit
Sensitivity
2.3 pg/mL
Range
93.8-6000 pg/mL
Reactivity
Mouse
Cat no : SE60026
Synonyms
Angiopoietin-1 receptor, CD202b, EC:2.7.10.1, Endothelial tyrosine kinase, HYK
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Tie-2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate, Tissue homogenates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 93.8-6000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21687 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 108% | 105%-114% |
| Cell culture supernatant | 93% | 90%-97% |
| Tissue homogenates | 98% | 94%-103% |
| Tissue lysate | 89% | 82%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,086.5 | 71.5 | 2.3 |
| 2 | 8 | 702.0 | 11.3 | 1.6 |
| 3 | 8 | 367.8 | 6.3 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3,066.4 | 72.1 | 2.4 |
| 2 | 16 | 703.0 | 23.9 | 3.4 |
| 3 | 16 | 358.6 | 14.3 | 4.0 |
Background Information
Tie-2 (also known as TEK or CD202b) is a receptor tyrosine kinase (RTK) critical for vascular development, angiogenesis, and endothelial cell function. Tie-2 is essential for blood vessel maturation. Genetic knockout of Tie-2 or Ang-1 in mice leads to embryonic lethality due to defects in cardiac development and vascular remodeling. Ligand-induced Tie-2 phosphorylation activates PI3K-AKT (promoting survival) and MAPK pathways (regulating proliferation and migration).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Angiopoietin-1 receptor, CD202b, EC:2.7.10.1, Endothelial tyrosine kinase, HYK |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
