Mouse SIGNR1/CD209b ELISA Kit
Sensitivity
2.5 pg/mL
Range
125-8000 pg/mL
Reactivity
Mouse
Cat no : KE10183
Synonyms
CD209, CD209 antigen-like protein B, Cd209b, DC-SIGNR1, DC-SIGN-related protein 1
Validation Data Gallery
Product Information
KE10183 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse SIGNR1/CD209b ELISA kit is to be used to detect and quantify protein levels of endogenous mouse SIGNR1/CD209b. The assay recognizes mouse SIGNR1/CD209b. An antibody specific for mouse SIGNR1/CD209b has been pre-coated onto the microwells. The mouse SIGNR1/CD209b protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse SIGNR1/CD209b is added to detect the captured mouse SIGNR1/CD209b protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse SIGNR1/CD209b ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.5 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 69165 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 86% | 77%-94% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,910.5 | 350.2 | 9.0 |
| 2 | 8 | 925.8 | 65.0 | 7.0 |
| 3 | 8 | 452.3 | 35.6 | 7.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3,814.4 | 322.9 | 8.5 |
| 2 | 16 | 932.2 | 65.7 | 7.0 |
| 3 | 16 | 469.4 | 41.8 | 8.9 |
Background Information
SIGNR1, also known as CD209b, is a type II transmembrane C-type lectin that plays a crucial role in immune recognition and phagocytosis. It is involved in the uptake of dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. SIGNR1 is physically associated with TLR4/MD2, which is suggested to play a role in the recognition of LPS. Additionally, SIGNR1 has been shown to bind directly to the complement C1 subcomponent, C1q, to assemble a non-conventional C3 convertase. In the context of cancer, SIGNR1 mediates IL-4-induced macrophage polarization, facilitating immune evasion in lung cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD209, CD209 antigen-like protein B, Cd209b, DC-SIGNR1, DC-SIGN-related protein 1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
