Mouse/Rat ALCAM ELISA Kit
Sensitivity
4.4 pg/mL
Range
39-2500 pg/mL
Reactivity
Mouse/Rat
Cat no : KE10159
Synonyms
Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen, Protein DM-GRASP
Validation Data Gallery
Product Information
KE10159 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat ALCAM ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat ALCAM. The assay recognizes mouse/rat ALCAM. An antibody specific for mouse/rat ALCAM has been pre-coated onto the microwells. The mouse/rat ALCAM protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse/rat ALCAM is added to detect the captured mouse/rat ALCAM protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat ALCAM ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.4 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11658 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 83% | 81%-87% |
| Tissue lysate | 102% | 97%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,418.4 | 81.3 | 5.7 |
| 2 | 8 | 378.0 | 22.0 | 5.8 |
| 3 | 8 | 114.7 | 6.8 | 5.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,471.4 | 85.0 | 5.8 |
| 2 | 16 | 376.3 | 21.3 | 5.7 |
| 3 | 16 | 113.1 | 5.9 | 5.2 |
Background Information
Mouse ALCAM (Activated Leukocyte Cell Adhesion Molecule, also known as CD166) is an immunoglobulin superfamily of cell adhesion molecules that is widely expressed in a variety of cell types including leukocytes, endothelial cells, and neuronal cells. It is involved in biological processes such as T-cell activation, immune synapse formation, cell migration and adhesion by interacting with molecules such as CD6. ALCAM plays an important role in the differentiation and self-renewal of hematopoietic stem cells in mice, and it is highly expressed in long term hematopoietic stem cells (LT-HSC), and the level increases with age. In addition, ALCAM also plays an important role in tumor progression and neurological development and disease, and is an important target for the study of immune regulation, tumor biology and neuroscience.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen, Protein DM-GRASP |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
