Mouse PDGFR beta ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.156-10 ng/mL
Reactivity
Mouse
Cat no : KE10166
Synonyms
Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140b, EC:2.7.10.1
Validation Data Gallery
Product Information
KE10166 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse PDGFR beta ELISA kit is to be used to detect and quantify protein levels of endogenous mouse PDGFR beta. The assay recognizes mouse PDGFR beta. An antibody specific for mouse PDGFR beta has been pre-coated onto the microwells. The mouse PDGFR beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse PDGFR beta is added to detect the captured mouse PDGFR beta protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse PDGFR beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18596 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 74%-108% |
| Cell culture supernatant | 97% | 90%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.84 | 0.35 | 5.95 |
| 2 | 8 | 1.20 | 0.05 | 3.99 |
| 3 | 8 | 0.66 | 0.04 | 5.47 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.69 | 0.55 | 9.72 |
| 2 | 16 | 1.21 | 0.06 | 5.02 |
| 3 | 16 | 0.65 | 0.04 | 6.64 |
Background Information
Mouse PDGFR beta (platelet-derived growth factor receptor beta) protein is a cell surface receptor tyrosine kinase involved in cell proliferation, differentiation, migration, and survival. PDGFR-β plays an important role in a variety of physiological and pathological processes, including tissue repair, angiogenesis, and tumor development. In obesity and diabetes studies, PDGFR-β has been associated with adipose tissue expansion and vascular remodeling. PDGF-BB secreted by macrophages was found to be an important regulator of the initiation phase of angiogenesis, which promotes adipose tissue neovascularization by causing pericytes to detach from the vasculature and promoting the germination and growth of adipose tissue neovascularization. In Alzheimer's disease (AD) studies, the PDGFR-β/TGF-β/Smad2/3 signaling pathway modulates blood-brain barrier (BBB) integrity and learning memory capacity. Exogenous PDGF-BB improved learning memory capacity, increased pericyte coverage in the hippocampus, enhanced TGF-βR1, p-Smad2, and p-Smad3 protein expression, and enhanced tight junction protein expression in an AD mouse model, thereby maintaining BBB integrity. These studies suggest that PDGFR-β has important research value in metabolic and neurodegenerative diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140b, EC:2.7.10.1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
