Speedy™ Mouse P-Selectin/CD62P One-Step ELISA Kit
Sensitivity
8.5 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse P-Selectin/CD62P One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 8.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20344 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 100% | 95%-110% |
| Cell culture supernatant | 96% | 89%-101% |
| Tissue lysate | 98% | 91%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,051.8 | 19.1 | 1.8 |
| 2 | 8 | 252.8 | 8.0 | 3.2 |
| 3 | 8 | 65.5 | 1.6 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,046.5 | 19.6 | 1.9 |
| 2 | 16 | 251.4 | 6.9 | 2.7 |
| 3 | 16 | 65.4 | 1.8 | 2.8 |
Background Information
P-sectieln, also named as SELP, GMP-140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. Soluble P-selectin is produced by platelets and endothelial cells and circulates in normal plasma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD62P, P-selectin, Grmp, P selectin, PADGEM |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
