Mouse MCP-1/CCL2 ELISA Kit
Validation Data Gallery
Product Information
KE10123 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse MCP-1. The assay recognizes mouse MCP-1. An antibody specific for mouse MCP-1 has been pre-coated onto the microwells. The mouse MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse MCP-1 is added to detect the captured mouse MCP-1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse MCP-1/CCL2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20296 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 110% | 79%-129% |
| Cell culture supernatant | 95% | 85%-117% |
| Tissue lysate | 100% | 92%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 509.8 | 10.8 | 2.1 |
| 2 | 20 | 119.1 | 3.2 | 2.7 |
| 3 | 20 | 29.1 | 1.5 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 508.0 | 33.1 | 6.5 |
| 2 | 24 | 122.5 | 6.6 | 5.4 |
| 3 | 24 | 29.8 | 1.9 | 6.2 |
Background Information
Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Mol Psychiatry Aspirin alleviates long-term high-fat diet-induced depressive-like behavior in male mice via suppressing arachidonic acid-mediated microglial activation and neuroinflammation. | ||
Aging Cell A Non-Channel Function of CFTR: Attenuating Mitochondrial Oxidative Stress and Cardiomyocyte Senescence via Stabilization by USP45. | ||
Biochem Pharmacol Targeting PIM1 ameliorates sepsis-associated acute kidney injury through inhibiting ferroptosis. | ||
Life Sci Inhibiting SIRT2 attenuates sepsis-associated acute kidney injury via NEU1 acetylation-mediated CD44/PI3K/AKT pathway activation. | ||
Eur J Pharmacol Glycin-proline-hydroxyproline modified N-terminal 20 peptide from CEMP1 blocks calcium ion influx to alleviate inflammatory response in gingival fibroblasts. |
