Mouse IgG2b isotype control Monoclonal antibody

Mouse IgG2b isotype control Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Megathura crenulata

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

11B8C4

Cat No : 66360-3-Ig

Print datasheet

Synonyms

Mouse IgG2b, Mouse IgG2b isotype control



经过测试的应用

Positive WB detected in
Positive IHC detected in
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66360-3-Ig targets Mouse IgG2b isotype control in WB, IHC, FC, chIP, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications chIP, WB
Tested Reactivity Megathura crenulata
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Mouse IgG2b isotype control
Synonyms Mouse IgG2b, Mouse IgG2b isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

实验方案

Product Specific Protocols
WB protocol for Mouse IgG2b isotype control antibody 66360-3-IgDownload protocol
IHC protocol for Mouse IgG2b isotype control antibody 66360-3-IgDownload protocol
FC protocol for Mouse IgG2b isotype control antibody 66360-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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ChIP

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WB

Aging Dis

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Immunity

Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity

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