Mouse IL-1R1 ELISA Kit
Sensitivity
29.1 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Cat no : KE10169
Synonyms
CD121 antigen-like family member A, CD121a, EC:3.2.2.6, Il1r1, IL-1R-1
Validation Data Gallery
Product Information
KE10169 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-1R1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-1R1. The assay recognizes mouse IL-1R1. An antibody specific for mouse IL-1R1 has been pre-coated onto the microwells. The mouse IL-1R1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-1R1 is added to detect the captured mouse IL-1R1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-1R1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 29.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16177 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 100% | 93%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,406.9 | 43.3 | 1.8 |
| 2 | 8 | 588.4 | 27.4 | 4.7 |
| 3 | 8 | 326.5 | 14.9 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,410.7 | 41.5 | 1.7 |
| 2 | 16 | 579.4 | 26.5 | 4.6 |
| 3 | 16 | 323.2 | 12.9 | 4.0 |
Background Information
The IL-1R family plays roles in the regulation of innate inflammation, host defense, and acquired immunity. Interleukin-1 receptor type 1 (IL-1R1) is expressed by all cells responsive to IL-1, the predominant type of IL-1R on T cells, fibroblasts, epithelial and endothelial cells. IL-1R1 activation promotes Ly6G expressing neutrophil accumulation in the injured kidney and generation of tumor necrosis factor (TNF) by intrarenal myeloid cells. The soluble form of IL-1R1 acts as a decoy inhibitor by capturing IL-1 cytokines in solution.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD121 antigen-like family member A, CD121a, EC:3.2.2.6, Il1r1, IL-1R-1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
