Speedy™ Mouse IL-13RA2 One-Step ELISA Kit
Sensitivity
0.04 ng/mL
Range
0.156-10 ng/mL
Reactivity
Mouse
Cat no : SE60011
Synonyms
CD213a2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, Il13ra2, IL-13R-alpha-2
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse IL-13RA2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue homogenates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16165 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 96% | 94%-98% |
| Tissue homogenates | 92% | 88%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.20 | 0.17 | 3.27 |
| 2 | 8 | 1.26 | 0.03 | 2.38 |
| 3 | 8 | 0.64 | 0.01 | 1.56 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.13 | 0.17 | 3.31 |
| 2 | 16 | 1.26 | 0.03 | 2.38 |
| 3 | 16 | 0.64 | 0.01 | 1.56 |
Background Information
IL-13RA2, also known as IL-13R and CD213a2, belongs to the type I cytokine receptor family. It binds as a monomer with high affinity to interleukin-13 (IL-13), but not to interleukin-4 (IL-4). IL-13RA2 lacks the cytoplasmic domain for signaling, indicating that it acts as a decoy receptor. IL13RA2 polymorphisms have been associated with systemic sclerosis. Overexpression of IL-13RA2 has been reported in several types of cancer, including melanoma, lung cancer, and brain tumor, which makes it a potential immunotherapeutic target.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD213a2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, Il13ra2, IL-13R-alpha-2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
