Mouse IL-12RB1 ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.78-50 ng/mL
Reactivity
Mouse
Cat no : KE10217
Synonyms
CD212, IL-12 receptor beta component, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, Il12rb
Validation Data Gallery
Product Information
KE10217 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-12RB1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-12RB1. The assay recognizes mouse IL-12RB1. An antibody specific for mouse IL-12RB1 has been pre-coated onto the microwells. The mouse IL-12RB1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-12RB1 is added to detect the captured mouse IL-12RB1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-12RB1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16161 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 115% | 107%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 24.66 | 1.33 | 5.38 |
| 2 | 8 | 6.05 | 0.45 | 7.49 |
| 3 | 8 | 3.07 | 0.12 | 3.78 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.95 | 1.77 | 6.82 |
| 2 | 16 | 6.19 | 0.46 | 7.48 |
| 3 | 16 | 3.12 | 0.15 | 4.88 |
Background Information
IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD212, IL-12 receptor beta component, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, Il12rb |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
