Speedy™ Mouse IGFBP3 One-Step ELISA Kit
Sensitivity
12.9 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Cat no : SE60012
Synonyms
IBP-3, IGF-binding protein 3, IGFBP-3, IGgfbp3, Insulin-like growth factor-binding protein 3
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse IGFBP3 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 12.9 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16009 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 97% | 89%-106% |
| Cell culture supernatant | 111% | 102%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,599.6 | 60.7 | 2.3 |
| 2 | 8 | 581.0 | 11.5 | 2.0 |
| 3 | 8 | 285.7 | 5.6 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,616.5 | 72.4 | 2.8 |
| 2 | 16 | 579.9 | 17.6 | 3.0 |
| 3 | 16 | 285.3 | 7.8 | 2.7 |
Background Information
Insulin-like growth factor binding protein 3, IGFBP3, is an N-linked glycosylated, phosphorylated, secretory protein with known antiproliferative and pro-apoptotic functions. IGFBP3 belongs to a family of high affinity insulin-like growth factor (IGF) binding proteins, which function to sequester extracellular IGF-1, preventing IGF-1 activation of the insulin-like growth factor receptor, IGF-1R. IGFBP3 is highly expressed in the endometrium and at the maternal-fetal interface in multiple species, including humans. In a mouse embryo coculture system,IGFBP3 was found to be secretedby endometrial cells that promote embryonic developmentby enhancing the effect of IGF.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IBP-3, IGF-binding protein 3, IGFBP-3, IGgfbp3, Insulin-like growth factor-binding protein 3 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
