Mouse CD79B ELISA Kit
Sensitivity
1.0 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Cat no : KE10152
Synonyms
B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, CD79b, Igb, Ig-beta
Validation Data Gallery
Product Information
KE10152 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD79B ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD79B. The assay recognizes mouse CD79B. An antibody specific for mouse CD79B has been pre-coated onto the microwells. The mouse CD79B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD79B is added to detect the captured mouse CD79B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD79B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate, Tissue homogenate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 15985 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue homogenate | 97% | 89%-105% |
| Tissue lysate | 95% | 90%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,759.4 | 78.0 | 2.8 |
| 2 | 8 | 824.6 | 19.2 | 2.3 |
| 3 | 8 | 232.2 | 21.3 | 9.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,681.4 | 131.2 | 4.9 |
| 2 | 16 | 769.8 | 61.5 | 8.0 |
| 3 | 16 | 230.1 | 20.8 | 9.0 |
Background Information
CD79 is composed of CD79a and CD79b components expressed almost exclusively on B cells and B-cell neoplasms. The transmembrane CD79a and CD79b proteins couple at the extracellular end with any one of the five different types of transmembrane Ig molecules (IgM, IgD, IgG, IgE, or IgA), which are disulfide-linked proteins composed of two Ig heavy chains and two Ig light chains. The intracytoplasmic ends of the CD79a/CD79b proteins can couple with intracellular signaling components as well. Furthermore, Activated B lymphocytes have increased amounts of the truncated CD79b. Loss of CD79b expression is a characteristic feature of CLL/SLL and may be of diagnostic use.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, CD79b, Igb, Ig-beta |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
