Mouse CD34 ELISA Kit
Sensitivity
1.7 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10138 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD34 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD34. The assay recognizes mouse CD34. An antibody specific for mouse CD34 has been pre-coated onto the microwells. The mouse CD34 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD34 is added to detect the captured mouse CD34 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD34 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12490 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 100% | 99%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 532.0 | 32.8 | 6.2 |
| 2 | 20 | 128.0 | 5.8 | 4.5 |
| 3 | 20 | 31.5 | 1.4 | 4.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 504.0 | 31.8 | 6.3 |
| 2 | 24 | 117.4 | 6.0 | 5.1 |
| 3 | 24 | 28.9 | 1.1 | 3.9 |
Background Information
CD34 is a 105- to 120-kDa transmembrane phosphoglycoprotein consisting of a cytoplasmic tail that contains PDZ-domain binding motifs, a single transmembrane helix and an extracellular domain that is heavily sialylated, O-linked glycosylated, and contains some N-linked glycosylation sites. It is expressed by the majority of hematopoietic stem/progenitor cells and also by a multitude of other nonhematopoietic cell types including muscle satellite cells, corneal keratocytes, interstitial cells, epithelial progenitors, and vascular endothelial cells. CD34 is widely used as a marker of vascular endothelial cells and hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cd34,CD34 antigen |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
