Mouse CD157 ELISA Kit
Sensitivity
6.2 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Mouse
Cat no : KE10162
Synonyms
ADP-ribosyl cyclase 2, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, Antigen BP3, Bone marrow stromal antigen 1, Bp3
Validation Data Gallery
Product Information
KE10162 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD157 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD157. The assay recognizes mouse CD157. An antibody specific for mouse CD157 has been pre-coated onto the microwells. The mouse CD157 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD157 is added to detect the captured mouse CD157 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD157 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue Lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 6.2 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12182 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 97% | 94%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,434.9 | 85.6 | 6.0 |
| 2 | 8 | 333.7 | 25.6 | 7.7 |
| 3 | 8 | 164.9 | 16.4 | 9.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,406.6 | 117.9 | 8.4 |
| 2 | 16 | 312.0 | 30.1 | 9.7 |
| 3 | 16 | 162.1 | 14.8 | 9.1 |
Background Information
CD157/BST1 glycosylphosphatidylinositol-anchored glycoprotein is an evolutionary conserved dual-function receptor and β-NAD+-metabolizing ectoenzyme of the ADP-ribosyl cyclases gene family. CD157 is a key player in regulating leukocyte adhesion and migration. It may also affect brain plasticity acting as a molecule at the crossroads of neurogenesis, cerebral angiogenesis, and immune regulation. Evaluation of soluble CD157 (sCD157) in pleural effusions provides a diagnostic aid in malignant mesothelioma. Furthermore, the levels of sCD157 correlate with human peripheral monocyte-derived macrophage bactericidal activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ADP-ribosyl cyclase 2, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, Antigen BP3, Bone marrow stromal antigen 1, Bp3 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
