Mouse BAFF ELISA Kit
Sensitivity
7.6 pg/mL
Range
125-8000 pg/mL
Reactivity
Mouse
Cat no : KE10182
Synonyms
B-cell-activating factor, CD257, Tnfsf13b, Tumor necrosis factor ligand superfamily member 13B, Tumor necrosis factor ligand superfamily member 13b, membrane form
Validation Data Gallery
Product Information
KE10182 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse BAFF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse BAFF. The assay recognizes mouse BAFF. An antibody specific for mouse BAFF has been pre-coated onto the microwells. The mouse BAFF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse BAFF is added to detect the captured mouse BAFF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse BAFF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.6 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24099 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 90%-104% | 97% |
| Cell culture supernatant | 87%-96% | 92% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4,317.4 | 193.4 | 4.5 |
| 2 | 8 | 1,028.0 | 44.4 | 4.3 |
| 3 | 8 | 544.8 | 30.8 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4,243.5 | 179.0 | 4.2 |
| 2 | 16 | 1,033.5 | 49.0 | 4.7 |
| 3 | 16 | 549.4 | 32.0 | 5.8 |
Background Information
TNFSF13B, also named as BAFF, BLYS, TALL1, TNFSF20, ZTNF4 and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13B is localized in chromosome region 13q32-q34 and is implicated in the regulation of immune response to infections, autoimmune disease, and inflammation. BAFF is a critical cytokine supporting the survival of naive B cells, acting through the BAFFR receptor. At the placental level, it is believed that TNFSF13B has an antiapoptotic effect. TNFSF13B is overexpressed in several autoimmune patients although the mechanism behind this feature is unclear.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B-cell-activating factor, CD257, Tnfsf13b, Tumor necrosis factor ligand superfamily member 13B, Tumor necrosis factor ligand superfamily member 13b, membrane form |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
