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Integrin Alpha 6 Polyclonal antibody

Integrin Alpha 6 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27189-1-AP

Print datasheet

Synonyms

CD49f, Integrin alpha 6, Integrin alpha-6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6



经过测试的应用

Positive WB detected inA549 cells, A431 cells, mouse skin tissue, rat lung tissue, HepG2 cells
Positive IHC detected inmouse skin tissue, human kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27189-1-AP targets Integrin Alpha 6 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, monkey, Hamster, bovine
Immunogen Integrin Alpha 6 fusion protein Ag25835 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha 6
Synonyms CD49f, Integrin alpha 6, Integrin alpha-6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6
Calculated Molecular Weight 1073 aa, 119 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC136456
Gene Symbol ITGA6
Gene ID (NCBI) 3655
RRIDAB_2880792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Integrin Alpha 6 antibody 27189-1-APDownload protocol
IHC protocol for Integrin Alpha 6 antibody 27189-1-APDownload protocol
FC protocol for Integrin Alpha 6 antibody 27189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Appl Mater Interfaces

A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells

Authors - Xinyang Zhao
bovineIF

Cells

Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak

Authors - Xiaolan Zhang
monkeyWB

Virulence

Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.

Authors - Yuchen Li
humanWB

Stem Cell Res Ther

Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Authors - Ling Leng
HamsterIF

J Virol

Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.

Authors - Xiu Xin
ratIF

Vascul Pharmacol

Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6

Authors - Li Wu