Human UTX ELISA Kit
Sensitivity
0.03 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human
Cat no : KE00693
Synonyms
[histone H3]-trimethyl-L-lysine(27) demethylase 6A, EC:1.14.11.68, Histone demethylase UTX, KDM6A, Lysine specific demethylase 6A
Validation Data Gallery
Product Information
KE00693 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human UTX ELISA kit is to be used to detect and quantify protein levels of endogenous human UTX. The assay recognizes human UTX. An antibody specific for human UTX has been pre-coated onto the microwells. The human UTX protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human UTX is added to detect the captured human UTX protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human UTX ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7403 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 114% | 101%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.91 | 0.53 | 4.86 |
| 2 | 8 | 2.29 | 0.12 | 5.04 |
| 3 | 8 | 1.16 | 0.03 | 2.91 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.70 | 0.51 | 4.76 |
| 2 | 16 | 2.27 | 0.09 | 4.18 |
| 3 | 16 | 1.15 | 0.06 | 5.12 |
Background Information
UTX protein, also known as KDM6A, is a histone demethylase whose main function is to remove the methylation modification of lysine 27 (H3K27) on histone H3, thereby activating the transcription of target genes and playing a vital role in epigenetic regulation. UTX plays an important role in embryonic development and cell differentiation, affecting cell fate determination and tissue-specific function by regulating the expression of specific genes. This protein is a component of the COMPASS-like complex, which includes the histone methyltransferase MLL4 (KMT2D) and other related proteins.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | [histone H3]-trimethyl-L-lysine(27) demethylase 6A, EC:1.14.11.68, Histone demethylase UTX, KDM6A, Lysine specific demethylase 6A |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
