Human Serpin F1/PEDF ELISA Kit
Sensitivity
0.12 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Cat no : KE00700
Synonyms
PEDF, PIG35, Cell proliferation-inducing gene 35 protein, EPC 1, EPC-1
Validation Data Gallery
Product Information
KE00700 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Serpin F1/PEDF ELISA kit is to be used to detect and quantify protein levels of endogenous human Serpin F1/PEDF. The assay recognizes human Serpin F1/PEDF. An antibody specific for human Serpin F1/PEDF has been pre-coated onto the microwells. The human Serpin F1/PEDF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Serpin F1/PEDF is added to detect the captured human Serpin F1/PEDF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Serpin F1/PEDF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.12 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5176 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 101% | 94%-109% |
| Cell culture supernatant | 102% | 93%-114% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.88 | 0.55 | 5.59 |
| 2 | 8 | 2.44 | 0.14 | 5.74 |
| 3 | 8 | 1.33 | 0.08 | 5.84 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.98 | 0.73 | 7.32 |
| 2 | 16 | 2.44 | 0.15 | 6.14 |
| 3 | 16 | 1.28 | 0.11 | 8.83 |
Background Information
Serpin F1, also known as Pigment Epithelium-Derived Factor (PEDF) or Cell Proliferation-Inducing Gene 35 Protein (PIG35), is a multifunctional protein that belongs to the serpin family. This family of proteins is characterized by their highly conserved core structure, which is critical for their function as serine protease inhibitors. Serpin F1 is secreted and has been shown to strongly inhibit angiogenesis, making it a potent inhibitor of blood vessel growth. It also has anti-tumor and neurotrophic functions, playing a significant role in various biological processes including cell differentiation, tumor suppression, and neuronal protection. Serpin F1 is expressed in quiescent cells and has been implicated in the regulation of gene expression and cellular proliferation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PEDF, PIG35, Cell proliferation-inducing gene 35 protein, EPC 1, EPC-1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
