Human STX1B ELISA Kit
Sensitivity
0.4 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00351 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human STX1B ELISA kit is to be used to detect and quantify protein levels of endogenous human STX1B. The assay recognizes human STX1B. An antibody specific for human STX1B has been pre-coated onto the microwells. The human STX1B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human STX1B is added to detect the captured human STX1B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human STX1B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 112755 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 87% | 81%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,012.3 | 54.9 | 5.4 |
| 2 | 20 | 229.3 | 9.8 | 4.3 |
| 3 | 20 | 56.7 | 5.0 | 8.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,036.7 | 84.4 | 8.1 |
| 2 | 24 | 246.8 | 20.9 | 8.5 |
| 3 | 24 | 54.5 | 5.3 | 9.7 |
Background Information
Syntaxins are a family of transmembrane proteins that belong to the SNARE complex. In conjunction with other SNAREs and the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways. Syntaxin 1A (STX1A) and syntaxin 1B (STX1B), two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release. STX1A and STX1B are expressed in neurons and are localized on the neuronal plasma membrane. Human STX1B shares 100% amino acid sequence identity with mouse and rat STX1B within the cytoplasmic domain.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | STX1B, STX1B1, STX1B2, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
