Human SP3 ELISA Kit
Sensitivity
0.07 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00859 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SP3 ELISA kit is to be used to detect and quantify protein levels of endogenous human SP3. The assay recognizes human SP3. An antibody specific for human SP3 has been pre-coated onto the microwells. The human SP3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SP3 is added to detect the captured human SP3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SP3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6670 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 84%-99% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.04 | 0.06 | 1.15 |
| 2 | 8 | 1.43 | 0.03 | 2.29 |
| 3 | 8 | 0.67 | 0.03 | 3.74 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.28 | 0.26 | 4.99 |
| 2 | 16 | 1.34 | 0.11 | 7.85 |
| 3 | 16 | 0.67 | 0.02 | 3.28 |
Background Information
Specificity Protein 3 (SP3) is a transcription factor belonging to the Sp1-related gene family. It regulates gene transcription by binding to GC and GT boxes in the regulatory elements of target genes. The SP3 protein contains a zinc-finger DNA-binding domain and multiple transcriptional activation domains, functioning as a dual-function transcription factor that can both activate and repress gene expression, depending on the cell type, target gene, and the presence of co-regulators. SP3 plays a crucial role in biological processes such as cell proliferation, differentiation, apoptosis, and embryonic development. Its abnormal expression is associated with various diseases, including Alzheimer's disease, breast cancer, and colorectal cancer. In some cases, SP3 may disrupt cell cycle regulation or inhibit apoptosis by competitively inhibiting the functions of other Sp family members (such as SP1). Additionally, SP3 is involved in the regulation of angiogenesis-related gene expression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SPR 2, SPR-2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
