Human S100A9 ELISA Kit
Sensitivity
17.6 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00671 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human S100A9 ELISA kit is to be used to detect and quantify protein levels of endogenous human S100A9. The assay recognizes human S100A9. An antibody specific for human S100A9 has been pre-coated onto the microwells. The human S100A9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human S100A9 is added to detect the captured human S100A9 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human S100A9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 17.6 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6280 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 91%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,229.2 | 82.9 | 6.7 |
| 2 | 8 | 337.5 | 29.7 | 8.8 |
| 3 | 8 | 171.8 | 15.1 | 8.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,280.8 | 121.5 | 9.5 |
| 2 | 16 | 353.1 | 34.4 | 9.7 |
| 3 | 16 | 182.5 | 17.3 | 9.5 |
Background Information
S100A9 (S100 calcium-binding protein A9) is also named as Calgranulin-B (CAGB), CFAG and MRP14. S100A9 is a member of the S100 protein family. It is a calcium‐binding protein that was first identified as a proinflammatory mediator released by bone marrow‐derived cells in response to cell damage, infection, or inflammation. S100A9 is also involved in carcinogenesis and tumor progression and inhibits the tumor immune response. S100A9 might activate the reactive oxygen species (ROS)‐dependent signaling pathways and protect against cell death. S100A9 acts as a scaffold recruiting ubiquitin specific peptidase 10 and phosphoglycerate mutase family member 5 (PGAM5) to form a tripolymer, causing the deubiquitination and stabilization of PGAM5, leading to mitochondrial fission and reactive oxygen species production, thereby promoting the growth and metastasis of HCC . The alarmins S100A8 and S100A9 are abundantly stored in neutrophils as the stable heterodimer S100A8/A9, also known as calprotectin, and are rapidly released in response to inflammatory stimuli and act as damage-associated molecular patterns.5 Neutrophils are the most important source of extracellular S100A8/A9.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit, CFAG |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
