Human RNASET2 ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00402 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RNASET2 ELISA kit is to be used to detect and quantify protein levels of endogenous human RNASET2. The assay recognizes human RNASET2. An antibody specific for human RNASET2 has been pre-coated onto the microwells. The human RNASET2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RNASET2 is added to detect the captured human RNASET2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RNASET2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8635 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 101% | 76%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5.06 | 0.15 | 3.00 |
| 2 | 20 | 1.12 | 0.03 | 2.30 |
| 3 | 20 | 0.32 | 0.01 | 4.22 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.84 | 0.21 | 4.34 |
| 2 | 24 | 1.07 | 0.03 | 2.55 |
| 3 | 24 | 0.30 | 0.01 | 3.73 |
Background Information
RNASET2 (Ribonuclease T2) is a highly conserved enzyme in a wide range of organisms and belongs to the RNase T2 family. The main biological role of intracellular RNASET2 is to cleave or degrade exogenous or endogenous RNA substrates as well as contributing to mitochondrial RNA metabolism, and also activating immune responses. In addition, it is involved in the regulation of angiogenesis, apoptosis, cell proliferation/differentiation, and immune regulation. In cancer, dysregulated expression of RNASET2 is associated with a variety of pathological conditions, including autoimmune diseases and cancer. The calculated molecular weight of human RNASET2 is approximately 35 kDa, but due to post-translational modifications such as glycosylation, its actual molecular weight may be observed as a mildly glycosylated form with a molecular weight of 38-45 kDa and a highly glycosylated form with a molecular weight of 50-80 kDa.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | RNase T2,RNASET2,bA514O12.3,EC:4.6.1.19,Ribonuclea |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
