Human PSIP1 ELISA Kit
Sensitivity
17.7 pg/mL
Range
93.8-6000 pg/mL
Reactivity
Human
Cat no : KE01008
Synonyms
CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF, Lens epithelium-derived growth factor
Validation Data Gallery
Product Information
KE01008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PSIP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human PSIP1. The assay recognizes human PSIP1. An antibody specific for human PSIP1 has been pre-coated onto the microwells. The human PSIP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PSIP1 is added to detect the captured human PSIP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PSIP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 17.7 pg/mL |
| Range | 93.8-6000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11168 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 111% | 95%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,103.0 | 196.5 | 6.3 |
| 2 | 8 | 690.4 | 58.0 | 8.4 |
| 3 | 8 | 402.9 | 33.5 | 8.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3115.1 | 232.4 | 7.5 |
| 2 | 16 | 686.8 | 54.2 | 7.9 |
| 3 | 16 | 396.3 | 32.3 | 8.2 |
Background Information
PSIP1 also known as LEDGF, is a crucial protein in human cells that functions primarily as a transcriptional co-activator. PSIP1 binds to methylated histone H3 lysine 36 (H3K36me) via the PWWP domain, and PSIP1 binding is enriched at the sites of RNAPII transcription. Two isoforms of PSIP1/LEDGF have been described, p75 and p52, which both contain the H3K36me2/3 binding PWWP domain. PSIP1 plays a central role in regulating gene expression, stress responses, and, most notably, is a critical co-factor for the human immunodeficiency virus (HIV). Its dual role in fundamental cellular processes and disease pathogenesis makes it a significant protein in molecular biology and medicine.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF, Lens epithelium-derived growth factor |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
