Human POMP ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.234-15 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00536 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human POMP ELISA kit is to be used to detect and quantify protein levels of endogenous human POMP. The assay recognizes human POMP. An antibody specific for human POMP has been pre-coated onto the microwells. The human POMP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human POMP is added to detect the captured human POMP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human POMP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.234-15 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51371 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 75% | 70%-77% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.67 | 0.44 | 5.76 |
| 2 | 8 | 1.97 | 0.08 | 3.99 |
| 3 | 8 | 0.90 | 0.05 | 6.06 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.51 | 0.47 | 6.31 |
| 2 | 16 | 1.85 | 0.17 | 9.39 |
| 3 | 16 | 0.89 | 0.06 | 6.96 |
Background Information
Proteasome maturation protein(POMP), is an essential factor of mammalian proteasome biogenesis. It facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function. A single nucleotide deletion in this gene causes an autosomal recessive skin disorder, keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) syndrome. POMP is strongly and consistently expressed from the basal to the granular layer of the epidermis in healthy people, whereas in KLICK patients, the staining is strong in the basal layer with a gradual decrease toward the granular layer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | POMP, C13orf12, HSPC014, HSPC036, hUMP1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
