Human/Mouse/Rat XDH ELISA Kit
Sensitivity
0.04 ng/mL
Range
0.47-30 ng/mL
Reactivity
Human/Mouse/Rat
Cat no : KE00902
Synonyms
xanthine dehydrogenase, Xanthine Oxidase, Xanthine oxidoreductase, XD, XDHA
Validation Data Gallery
Product Information
KE00902 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat XDH ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat XDH. The assay recognizes human/mouse/rat XDH. An antibody specific for human/mouse/rat XDH has been pre-coated onto the microwells. The human/mouse/rat XDH protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat XDH is added to detect the captured human/mouse/rat XDH protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat XDH ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7498 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 83% | 71%-93% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 13.81 | 0.55 | 3.96 |
| 2 | 8 | 3.82 | 0.12 | 3.13 |
| 3 | 8 | 2.05 | 0.08 | 3.68 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.51 | 0.96 | 6.62 |
| 2 | 16 | 3.91 | 0.15 | 3.89 |
| 3 | 16 | 2.05 | 0.08 | 3.93 |
Background Information
XDH, also named as XDHA, XO, and XD, belongs to the xanthine dehydrogenase family. It catalyzes the last two steps of purine catabolism in man, the formation of the end product uric acid from hypoxanthine and xanthine. The mammalian enzyme is synthesized as a dehydrogenase (XDH), which uses NAD as the electron acceptor, but it can be converted into an oxidase (XO) both in vivo and in vitro. This gene encodes a protein of 150 kDa, and the 150 kDa polypeptide can be processed into a fragment of 130 kDa and further to 85 kDa by spontaneous proteolytic cleavage. In addition, the full-length polypeptide can also be partially processed into 87 kDa and 59 kDa fragments. It can also exist as a homodimer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | xanthine dehydrogenase, Xanthine Oxidase, Xanthine oxidoreductase, XD, XDHA |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
