Human/Mouse/Rat Noggin ELISA Kit
Sensitivity
1.0 pg/mL
Range
125-8000 pg/mL
Reactivity
Human/Mouse/Rat
Validation Data Gallery
Product Information
KE00563 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat Noggin ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat Noggin. The assay recognizes human/mouse/rat Noggin. An antibody specific for human/mouse/rat Noggin has been pre-coated onto the microwells. The human/mouse/rat Noggin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat Noggin is added to detect the captured human/mouse/rat Noggin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat Noggin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9241 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 72% | 72%-73% |
| Tissue lysate | 78% | 71%-91% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4,879.9 | 340.2 | 7.0 |
| 2 | 8 | 1,113.2 | 52.3 | 4.7 |
| 3 | 8 | 530.9 | 26.5 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5,059.9 | 423.9 | 8.4 |
| 2 | 16 | 1,105.2 | 55.0 | 5.0 |
| 3 | 16 | 533.7 | 28.9 | 5.4 |
Background Information
Noggin is a secreted protein that belongs to the BMP antagonist family, and it has been found to play a complex and context-dependent role in cancer development (PMID: 38012621). Noggin has been shown to have anti-cancer effects by inhibiting the activity of BMPs, which are a group of proteins that belong to the TGF-β superfamily and are involved in various cellular processes such as cell proliferation, differentiation, and migration (PMID: 32486027). By blocking BMP activity, Noggin has been shown to inhibit the growth of osteolytic prostate cancer lesions (PMID: 16126463). In lung cancer cells, Noggin abrogated angiogenic activity enhanced by BMP-2 (PMID: 15037653). However, Noggin can also have pro-cancer effects in certain contexts. For example, in patients with gastric cancer, high Noggin expression is associated with a poor prognosis, and Noggin promotes the proliferation of gastric cancer cells by upregulating EGFR signaling (PMID: 32705152).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NOG, Noggin, SYM1, SYNS1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
