Human/Mouse/Rat GSR ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human/Mouse/Rat
Validation Data Gallery
Product Information
KE00840 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat GSR ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat GSR. The assay recognizes human/mouse/rat GSR. An antibody specific for human/mouse/rat GSR has been pre-coated onto the microwells. The human/mouse/rat GSR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat GSR is added to detect the captured human/mouse/rat GSR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat GSR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2936 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 81% | 71%-90% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.29 | 0.24 | 2.63 |
| 2 | 8 | 3.18 | 0.07 | 2.17 |
| 3 | 8 | 1.84 | 0.06 | 3.42 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 8.76 | 0.62 | 7.06 |
| 2 | 16 | 2.95 | 0.25 | 8.32 |
| 3 | 16 | 1.82 | 0.06 | 3.18 |
Background Information
GSR (glutathione-disulfide reductase) is also named as glutathione reductase (GR), GLUR, GRD1, which is an enzyme that catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form of glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. Glutathione plays a key role in maintaining proper function and preventing oxidative stress by acting as a scavenger for hydroxyl radicals, singlet oxygen, and various electrophiles. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase, which in turn reduces H2O2, a dangerously reactive species within the cell. In addition, GSH plays a key role in the metabolism and clearance of xenobiotics, acts as a cofactor in certain detoxifying enzymes, and regenerates antioxidants such as vitamins C and E to their reactive forms. The ratio of GSSG/GSH present in the cell is a key factor in maintaining the oxidative cell balance, where it is critical that a cell maintains high levels of the reduced glutathione and a low level of oxidized glutathione disulfide. This narrow balance is "buffered" by GSR, which catalyzes the reduction of GSSG to GSH.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:1.8.1.7, GLUR, glutathione reductase, GRase, GRD1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
